Prof. Dr. rer. nat. Oliver Seitz

Profil

• Basenlabile Auxiliare für die cysteinfreie Peptidverknüpfung

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 09/2010 - 04/2015 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• DFG-Sachbeihilfe: Energietransfer bei erzwungener Interkalation: Responsive und zugleich helle DNA-basierte Hochleistungssonden für die RNA-Visualisierung in lebenden Zellen

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 09/2013 - 06/2017 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• DFG-Sachbeihilfe: Hochleistungs-Auxiliare für eine cysteintolerante native chemische Peptidverknüpfung an beliebigen Aminosäuren

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 01/2018 - 12/2021 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• DNA-katalysierte Verknüpfungs-Cyclisierungs-Reaktionen: Entwicklung einer hochselektiven, signalamplifizierenden Methode für die Mutationsanalyse

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 09/2007 - 10/2011 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Duplex-Oligodesoxynucleotide mit C-glycosidisch gebundenen Basensurrogaten zur Untersuchung des Basen-Ausklapp-Mechanismus und selektiven Inhibition von DNA-Methyltransferasen I

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 01/2005 - 12/2008 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Duplex-Oligodesoxynucleotide mit C-glycosidisch gebundenen Basensurrogaten zur Untersuchung des Basen-Ausklapp-Mechanismus und selektiven Inhibition von DNA-Methyltransferasen II

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 09/2007 - 10/2008 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Eine allgemeine Methode zur Fmoc-basierten Festphasensynthese von Peptidthioestern über S-S-Acyltransfer

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 06/2009 - 03/2013 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Energietransfer bei erzwungener Interkalation. Neue Hochleistungssonden für die in vitro und in vivo Echtzeit-Nucleinsäure-Detektion

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 12/2007 - 05/2012 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• EU: Application of Novel Cyclic Ligation Auxiliaries in the Study of Transthyretin and Derived Pathologies (CycLATTR)

  Quelle ↗

  Zeitraum: 02/2013 - 01/2015 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• EU: RNA-Codified Release of Cytotoxic Peptides From Pna Prodrugs as New Therapeutic Approach to Cancer (Molecular doctors)

  Quelle ↗

  Zeitraum: 04/2011 - 03/2013 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Fluorogene Peptid-Ligation für Proteinabbildungen in lebenden Zellen

  Quelle ↗

  Förderer: Wirtschaftsunternehmen / gewerbliche Wirtschaft Zeitraum: 07/2013 - 06/2014 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• GRK 2473/1: Bioaktive Peptide – Innovative Aspekte zur Synthese und Biosynthese

  Quelle ↗

  Förderer: DFG Graduiertenkolleg Zeitraum: 04/2019 - 09/2023 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• GRK 2473 / 2: Bioaktive Peptide – Innovative Aspekte zur Synthese und Biosynthese

  Quelle ↗

  Förderer: DFG Graduiertenkolleg Zeitraum: 01/2024 - 03/2028 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• GRK 2473: Bioaktive Peptide - Innovative Aspekte zur Synthese und Biosynthese

  Quelle ↗

  Förderer: DFG Graduiertenkolleg Zeitraum: 04/2019 - 03/2028 Projektleitung: Prof. Dr. Roderich Süssmuth

• Leibniz-Humboldt-Professur "Chemische Biologie"

  Quelle ↗

  Förderer: Einstein Stiftung Berlin Zeitraum: 03/2012 - 02/2017 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Molecular Diagnostics; Overcoming target inhibition in nucleic acid-templated ligation reactions

  Quelle ↗

  Zeitraum: 07/2012 - 12/2013 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Nucleinsäurehybridisierungsgesteuerte Kontrolle der Peptidkonformation als Werkzeug zum Studium der biologischen Signaltransduktion

  Quelle ↗

  Förderer: Volkswagen Stiftung Zeitraum: 11/2003 - 11/2006 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Reactions That Translate mRNA Into Dug-Like Molecules (TRIGGDRUG)

  Quelle ↗

  Förderer: Horizon 2020: ERC Advanced Grant Zeitraum: 01/2016 - 12/2020 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• RNA-gesteuerte Peptidsynthese über Peptidyltransferreaktionen an Peptid-Nucleinsäurekonjugaten

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 01/2013 - 06/2016 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Second sino-german symposium on chemical biology (Veranstaltung: 03.12.-08.12.06, Berlin)

  Quelle ↗

  Zeitraum: 11/2006 - 12/2006 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Selbstreinigende Fmoc-Festphasensynthese C-terminaler Peptidthioester

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 01/2006 - 12/2007 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Selbstreinigende Synthese von Peptidthioestern zum Aufbau von Proteindomänen auf Arrays und Beads

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 07/2008 - 12/2012 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• SFB 1449/2: Entwicklung, Charakterisierung und Anwendung von Nanosonden für fortgeschrittene FLIM-Studien an synthetischen und zellulären Hydrogelen (TP A04)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 01/2025 - 12/2028 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• SFB 1449/2: Semi-synthetische Ansätze zur Untersuchung der Funktion von Muzinen (TP C01)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 01/2025 - 12/2028 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• SFB 765/3: Nucleinsäure-Konjugate als selbstorganisierte Template für die multivalente Ligandenpräsentation (TP B01)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 01/2008 - 12/2019 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Single molecule RNA biology - dynamics and function of RNA from transcription to degradation

  Quelle ↗

  Förderer: Einstein Stiftung Berlin Zeitraum: 12/2013 - 11/2016 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Skalierbare, parallele & nachhaltige HPLC-freie Reinigung von Peptiden durch eine chemische Affinitätschromatographie

  Quelle ↗

  Förderer: Bundesministerium für Wirtschaft und Energie Zeitraum: 09/2016 - 04/2018 Projektleitung: Dipl.-Chem. Robert Zitterbart, Prof. Dr. rer. nat. Oliver Seitz

• SPP1623/1: Selective bioconjugation of proteins in live cells via peptide-directed acyl and alkyl transfer reactions

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 12/2012 - 01/2016 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• SPP 1623: Peptidtemplat-gesteuerte Biokonjugation an Proteinen in und auf lebenden Zellen für Untersuchungen von GPCR-Transport und -Kreuzreaktivität

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 02/2016 - 06/2019 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• SPP 1784: Zelluläre Bildgebung der RNA-C-zu-U-Editierung

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 01/2016 - 06/2019 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Steuerung und Katalyse der chemischen Verknüpfung von PNA-Konjugaten durch Oligonucleotid-Template I

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 09/2003 - 02/2006 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Steuerung und Katalyse der chemischen Verknüpfung von PNA-Konjugaten durch Oligonucleotid-Template II

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 11/2005 - 08/2007 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Synthese und Modifizierung von Biopolymeren

  Quelle ↗

  Zeitraum: 08/2003 - 07/2008 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Testung und Weiterentwicklung von fluoreszenten Sonden

  Quelle ↗

  Zeitraum: 04/2006 - 09/2006 Projektleitung: Prof. Dr. rer. nat. Oliver Seitz

• Asociación Centro de Investigación Cooperativa en Nanociencia – CIC nanoGUNE

  PT

  150 Treffer62.0%
  • DYnamic control in hybrid plasmonic NAnopores: road to next generation multiplexed single MOlecule detection
    P

    62.0%

• Scriba Nanotecnologie SRL

  PT

  69 Treffer58.2%
  • Integrated Self-Assembled SWITCHable Systems and Materials: Towards Responsive Organic Electronics – A Multi-Site Innovative Training Action (iSwitch)
    P

    58.2%

• BASF SE

  PT

  98 Treffer58.2%
  • Integrated Self-Assembled SWITCHable Systems and Materials: Towards Responsive Organic Electronics – A Multi-Site Innovative Training Action (iSwitch)
    P

    58.2%
  • SIB-DE_Forschung - Sodium-Ion-Battery Deutschland (SIB:DE Initiative) - Eignung der Natrium-Ionen-Technologie für die europäische Energie- und Mobilitätswende
    T

    52.4%

• A.P.E. Research srl

  PT

  153 Treffer58.2%
  • Integrated Self-Assembled SWITCHable Systems and Materials: Towards Responsive Organic Electronics – A Multi-Site Innovative Training Action (iSwitch)
    P

    58.2%
  • EU: Bottom-Up Generation of atomicalLy Precise syntheTIc 2D MATerials for High Performance in Energy and Electronic Applications – A Multi-Site Innovative Training Action (ULTIMATE)
    P

    55.6%

• InnovationLab GmbH

  P

  100 Treffer58.0%
  • Interfaces in opto-electronic thin film multilayer devices
    P

    58.0%

• Bernstein Center for Computational Neuroscience Berlin

  PT

  75 Treffer56.8%
  • DFG-Sachbeihilfe: Aufmerksamkeit und sensorische Integration im aktiven Sehen von bewegten Objekten
    T

    56.8%
  • SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene
    P

    48.8%

• Science & Startups Berlin

  T

  30 Treffer55.6%
  • Zuwendung im Rahmen des Programms „exist – Existenzgründungen aus der Wissenschaft“ aus dem Bundeshaushalt, Einzelplan 09, Kapitel 02, Titel 68607, Haushaltsjahr 2026, sowie aus Mitteln des Europäischen Strukturfonds (hier Euro-päischer Sozialfonds Plus – ESF Plus) Förderperiode 2021-2027 – Kofinanzierung für das Vorhaben: „exist Women“
    T

    55.6%

• International Business Machines Corporation Research GmbH

  PT

  145 Treffer55.6%
  • EU: Bottom-Up Generation of atomicalLy Precise syntheTIc 2D MATerials for High Performance in Energy and Electronic Applications – A Multi-Site Innovative Training Action (ULTIMATE)
    P

    55.6%
  • EU: Simulation in Multiscale Physical and Biological Systems (STIMULATE)
    P

    54.6%

• Graphenea S.A.

  PT

  77 Treffer55.6%
  • EU: Bottom-Up Generation of atomicalLy Precise syntheTIc 2D MATerials for High Performance in Energy and Electronic Applications – A Multi-Site Innovative Training Action (ULTIMATE)
    P

    55.6%

• Istituto Italiano di Tecnologia

  PT

  77 Treffer55.6%
  • EU: Bottom-Up Generation of atomicalLy Precise syntheTIc 2D MATerials for High Performance in Energy and Electronic Applications – A Multi-Site Innovative Training Action (ULTIMATE)
    P

    55.6%

• Multivalency as a Chemical Organization and Action Principle

  2012

  Angewandte Chemie International Edition · 985 Zitationen · DOI

  Multivalent interactions can be applied universally for a targeted strengthening of an interaction between different interfaces or molecules. The binding partners form cooperative, multiple receptor-ligand interactions that are based on individually weak, noncovalent bonds and are thus generally reversible. Hence, multi- and polyvalent interactions play a decisive role in biological systems for recognition, adhesion, and signal processes. The scientific and practical realization of this principle will be demonstrated by the development of simple artificial and theoretical models, from natural systems to functional, application-oriented systems. In a systematic review of scaffold architectures, the underlying effects and control options will be demonstrated, and suggestions will be given for designing effective multivalent binding systems, as well as for polyvalent therapeutics.

• Hydrothermal Synthesis of Graphene-TiO₂ Nanotube Composites with Enhanced Photocatalytic Activity

  2012

  ACS Catalysis · 954 Zitationen · DOI

  In this study, TiO2 nanotube (TNT)/reduced graphene oxide (hGO) composites were prepared by an alkaline hydrothermal process. This was achieved by decorating graphene oxide (GO) layers with commercially available TiO2 nanoparticles (P90) followed by hydrothermal synthesis, which converts the TiO2 nanoparticles to small diameter (∼9 nm) TNTs on the hGO surface. The alkaline medium used to synthesize the TNTs simultaneously converts GO to deoxygenated graphene oxide (hGO). Compared to GO, the hGO has a ∼70% reduction of oxygenated species after alkaline hydrothermal treatment. The graphene nature of hGO in the composites was confirmed by X-ray diffraction (XRD), Raman, FTIR, and X-ray photoelectron spectroscopy (XPS) analysis. The photocatalytic performance of the hGO-TNT composites was evaluated for the photodegradation of malachite green. It was found that the ratio of hGO to TNT in the composites significantly affects the photocatalytic activity. Higher amounts of hGO in hGO-TNT composites showed lower photocatalytic activity than pure TNTs. The composite with 10% hGO showed the highest photocatalytic activity, with a 3-fold enhancement in photocatalytic efficiency over pure TNTs. It is expected that the synthesis of “high surface area-small diameter” TiO2 nanotubes and simultaneous conversion of GO to graphene like hGO “without using strong reducing agents” could be a promising strategy for preparing other types of carbon based TiO2 nanotube composite photocatalysts.

• Native Chemical Ligation at Valine

  2008

  Angewandte Chemie International Edition · 341 Zitationen · DOI

  Peptide ligation at hydrophobic sites is possible with a ligation–desulfurization strategy in which penicillamine serves as a precursor of valine. The β,β-dimethylcysteine peptides reacted surprisingly fast in native chemical ligation reactions. Even the sterically crowded and unpolar Leu–Val bond can be formed in high yield.

• Vanadium Oxide Nanowire–Carbon Nanotube Binder‐Free Flexible Electrodes for Supercapacitors

  2011

  Advanced Energy Materials · 331 Zitationen · DOI

  Abstract Vanadium pentoxide (V 2 O 5 ) layered nanostructures are known to have very stable crystal structures and high faradaic activity. The low electronic conductivity of V 2 O 5 greatly limits the application of vanadium oxide as electrode materials and requires combining with conducting materials using binders. It is well known that the organic binders can degrade the overall performance of electrode materials and need carefully controlled compositions. In this study, we develop a simple method for preparing freestanding carbon nanotube (CNT)‐V 2 O 5 nanowire (VNW) composite paper electrodes without using binders. Coin cell type (CR2032) supercapacitors are assembled using the nanocomposite paper electrode as the anode and high surface area carbon fiber electrode (Spectracarb 2225) as the cathode. The supercapacitor with CNT‐VNW composite paper electrode exhibits a power density of 5.26 kW Kg −1 and an energy density of 46.3 Wh Kg −1 . (Li)VNWs and CNT composite paper electrodes can be fabricated in similar manner and show improved overall performance with a power density of 8.32 kW Kg −1 and an energy density of 65.9 Wh Kg −1 . The power and energy density values suggest that such flexible hybrid nanocomposite paper electrodes may be useful for high performance electrochemical supercapacitors.

• Tumour-infiltrating lymphocytes predict response to definitive chemoradiotherapy in head and neck cancer

  2013

  British Journal of Cancer · 301 Zitationen · DOI

  The positive correlation between a high number of infiltrating CD3+ and CD8+ cells and clinical outcome indicates that TILs may have a beneficial role in HNSCC patients and may serve as a biomarker to identify patients likely to benefit from definitive CRT.

• Forced Intercalation Probes (FIT Probes): Thiazole Orange as a Fluorescent Base in Peptide Nucleic Acids for Homogeneous Single‐Nucleotide‐Polymorphism Detection

  2004

  ChemBioChem · 232 Zitationen · DOI

  Fluorescent base analogues in DNA are versatile probes of nucleic acid-nucleic acid and nucleic acid-protein interactions. New peptide nucleic acid (PNA) based probes are described in which the intercalator dye thiazole orange (TO) serves as a base surrogate. The investigation of six TO derivatives revealed that the linker length and the conjugation site decided whether a base surrogate conveys sequence-selective DNA binding and whether fluorescence is increased or decreased upon single-mismatched hybridization. One TO derivative conferred universal PNA-DNA base pairing while maintaining duplex stability and hybridization selectivity. TO fluorescence increased up to 26-fold upon hybridization. In contrast to most other probes, in which fluorescence is invariant once hybridization had occurred, the emission of TO-containing PNA probes is attenuated when forced to intercalate next to a mismatched base pair. The specificity of DNA detection is therefore not limited by the selectivity of probe-target binding and a DNA target can be distinguished from its single-base mutant under nonstringent hybridization conditions. This property should be of advantage for real-time quantitative PCR and nucleic acid detection within living cells.

• Dissecting the role of protein phosphorylation: a chemical biology toolbox

  2022

  Chemical Society Reviews · 231 Zitationen · DOI

  Protein phosphorylation is a crucial regulator of protein and cellular function, yet, despite identifying an enormous number of phosphorylation sites, the role of most is still unclear. Each phosphoform, the particular combination of phosphorylations, of a protein has distinct and diverse biological consequences. Aberrant phosphorylation is implicated in the development of many diseases. To investigate their function, access to defined protein phosphoforms is essential. Materials obtained from cells often are complex mixtures. Recombinant methods can provide access to defined phosphoforms if site-specifically acting kinases are known, but the methods fail to provide homogenous material when several amino acid side chains compete for phosphorylation. Chemical and chemoenzymatic synthesis has provided an invaluable toolbox to enable access to previously unreachable phosphoforms of proteins. In this review, we selected important tools that enable access to homogeneously phosphorylated protein and discuss examples that demonstrate how they can be applied. Firstly, we discuss the synthesis of phosphopeptides and proteins through chemical and enzymatic means and their advantages and limitations. Secondly, we showcase illustrative examples that applied these tools to answer biological questions pertaining to proteins involved in signal transduction, control of transcription, neurodegenerative diseases and aggregation, apoptosis and autophagy, and transmembrane proteins. We discuss the opportunities and challenges in the field.

• Glycopeptide Synthesis and the Effects of Glycosylation on Protein Structure and Activity

  2000

  ChemBioChem · 213 Zitationen · DOI

  Despite the omnipresence of protein glycosylation in nature, little is known about how the attachment of carbohydrates affects peptide and protein activity. One reason is the lack of a straightforward method to access biologically relevant glycopeptides and glycoproteins. The isolation of homogeneous glycopeptides from natural sources is complicated by the heterogeneity of naturally occuring glycoproteins. It is chemical and chemoenzymatic synthesis that is meeting the challenge to solve this availability problem, thus playing a key role for the advancement of glycobiology. The current art of glycopeptide synthesis, albeit far from being routine, has reached a level of maturity that allows for the access to homogeneous and pure material for biological and medicinal research. Even the ambitious goal of the total synthesis of an entire glycoprotein is within reach. It is demonstrated that with the help of synthetic glycopeptides the effects of glycosylation on protein structure and function can be studied in molecular detail. For example, in immunology, synthetic (tumour-specific) glycopeptides can be used as immunogens to elicit a tumour-cell-specific immune response. Again, synthetic glycopeptides are an invaluable tool to determine the fine specificity of the immune response that can be mediated by both carbohydrate-specific B and T cells. Furthermore, selected examples for the use of synthetic glycopeptides as ligands of carbohydrate-binding proteins and as enzyme substrates or inhibitors are presented.

• 9‐Fluorenylmethoxycarbonyl‐Based Solid‐Phase Synthesis of Peptide α‐Thioesters

  2010

  Angewandte Chemie International Edition · 192 Zitationen · DOI

  Peptide thioesters play a key role in convergent protein synthesis strategies such as native chemical ligation, traceless Staudinger ligation, and Ag(+) -mediated thioester ligation. The Boc-based solid-phase synthesis provides a very reliable access to peptide thioesters. However, the acid lability of many peptide modifications and the requirements of most parallel peptide synthesizers call for the milder Fmoc-based solid-phase synthesis. The Fmoc-based synthesis of peptide thioesters is more cumbersome and typically proceeds with lower yields than the synthesis of peptide acids and peptide amides. The success of native chemical ligation and related technologies has sparked intensive research effort devoted to the development of new methods. The recent progress in this rapidly expanding field is reviewed.

• Peptide-tags for site-specific protein labelling <i>in vitro</i> and <i>in vivo</i>

  2016

  Molecular BioSystems · 185 Zitationen · DOI

  Peptide-tag based labelling can be achieved by (i) enzymes (ii) recognition of metal ions or small molecules and (iii) peptide-peptide interactions and enables site-specific protein visualization to investigate protein localization and trafficking.

• Infrared Characterization of Interfacial Si−O Bond Formation on Silanized Flat SiO₂/Si Surfaces

  2010

  Langmuir · 177 Zitationen · DOI

  Chemical functionalization of silicon oxide (SiO(2)) surfaces with silane molecules is an important technique for a variety of device and sensor applications. Quality control of self-assembled monolayers (SAMs) is difficult to achieve because of the lack of a direct measure for newly formed interfacial Si-O bonds. Herein we report a sensitive measure of the bonding interface between the SAM and SiO(2), whereby the longitudinal optical (LO) phonon mode of SiO(2) provides a high level of selectivity for the characterization of newly formed interfacial bonds. The intensity and spectral position of the LO peak, observed upon silanization of a variety of silane molecules, are shown to be reliable fingerprints of formation of interfacial bonds that effectively extend the Si-O network after SAM formation. While the IR absorption intensities of functional groups (e.g., >C=O, CH(2)/CH(3)) depend on the nature of the films, the blue-shift and intensity increase of the LO phonon mode are common to all silane molecules investigated and their magnitude is associated with the creation of interfacial bonds only. Moreover, results from this study demonstrate the ability of the LO phonon mode to analyze the silanization kinetics of SiO(2) surfaces, which provides mechanistic insights on the self-assembly process to help achieve a stable and high quality SAM.

• Multivalenz als chemisches Organisations‐ und Wirkprinzip

  2012

  Angewandte Chemie · 176 Zitationen · DOI

  Abstract Multivalente Wechselwirkungen können universell zur gezielten Bindungsverstärkung zwischen verschiedenen Grenzflächen oder Molekülen genutzt werden. Die Bindungspartner bilden dabei kooperativ multiple Rezeptor‐Ligand‐Wechselwirkungen, die auf einzelnen schwachen, nichtkovalenten Bindungen basieren und daher prinzipiell reversibel sind. Daher spielen multi‐ und polyvalente Wechselwirkungen in biologischen Systemen eine entscheidende Rolle für Erkennungs‐, Adhäsions‐ und Signalprozesse. Die wissenschaftliche und praktische Etablierung dieses Prinzips wird anhand der Entwicklung von natürlichen Systemen über einfache artifizielle und theoretische Modelle hin zu anwendungsorientierten funktionalen Systemen demonstriert. Anhand eines systematischen Überblicks über Gerüstarchitekturen werden die zugrunde liegenden Wirk‐ und Steuerungsmöglichkeiten aufgezeigt und Designvorschläge für möglichst effektive multivalente Bindungspartner bis hin zu polyvalenten Therapeutika aufgezeigt.

• Adult Palatum as a Novel Source of Neural Crest-Related Stem Cells

  2009

  Stem Cells · 160 Zitationen · DOI

  Somatic neural and neural crest stem cells are promising sources for cellular therapy of several neurodegenerative diseases. However, because of practical considerations such as inadequate accessibility of the source material, the application of neural crest stem cells is strictly limited. The secondary palate is a highly regenerative and heavily innervated tissue, which develops embryonically under direct contribution of neural crest cells. Here, we describe for the first time the presence of nestin-positive neural crest-related stem cells within Meissner corpuscles and Merkel cell-neurite complexes located in the hard palate of adult Wistar rats. After isolation, palatal neural crest-related stem cells (pNC-SCs) were cultivated in the presence of epidermal growth factor and fibroblast growth factor under serum-free conditions, resulting in large amounts of neurospheres. We used immunocytochemical techniques and reverse transcriptase-polymerase chain reaction to assess the expression profile of pNC-SCs. In addition to the expression of neural crest stem cell markers such as Nestin, Sox2, and p75, we detected the expression of Klf4, Oct4, and c-Myc. pNC-SCs differentiated efficiently into neuronal and glial cells. Finally, we investigated the potential expression of stemness markers within the human palate. We identified expression of stem cell markers nestin and CD133 and the transcription factors needed for reprogramming of somatic cells into pluripotent cells: Sox2, Oct4, Klf4, and c-Myc. These data show that cells isolated from palatal rugae form neurospheres, are highly plastic, and express neural crest stem cell markers. In addition, pNC-SCs may have the ability to differentiate into functional neurons and glial cells, serving as a starting point for therapeutic studies.

• Triplex Molecular Beacons as Modular Probes for DNA Detection

  2007

  Angewandte Chemie International Edition · 146 Zitationen · DOI

  Reporting live: Triplex formation is used to construct a stem–loop probe, which is opened upon binding of the DNA target (red). The triplex molecular beacon is composed of a single DNA strand (blue), a stem-forming oligomer (black), and is labeled with a fluorophore and a quencher (red and blue circles). This concept facilitates the introduction of further functionalities such as additional quenchers in the assembly of “superquenched” beacons. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z700289_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

• Importance of Monolayer Quality for Interpreting Current Transport through Organic Molecules:  Alkyls on Oxide-Free Si

  2006

  Langmuir · 146 Zitationen · DOI

  We study the effect of monolayer quality on the electrical transport through n-Si/C(n)H(2n+1)/Hg junctions (n = 12, 14, and 18) and find that truly high quality layers and only they, yield the type of data, reported by us in Phys. Rev. Lett. 2005, 95, 266807, data that are consistent with the theoretically predicted behavior of a Schottky barrier coupled to a tunnel barrier. By using that agreement as our starting point, we can assess the effects of changing the quality of the alkyl monolayers, as judged from ellipsometer, contact angle, XPS, and ATR-FTIR measurements, on the electrical transport. Although low monolayer quality layers are easily identified by one or more of those characterization tools, as well as from the current-voltage measurements, even a combination of characterization techniques may not suffice to distinguish between monolayers with minor differences in quality, which, nevertheless, are evident in the transport measurement. The thermionic emission mechanism, which in these systems dominates at low forward bias, is the one that is most sensitive to monolayer quality. It serves thus as the best quality control. This is important because, even where tunneling characteristics appear rather insensitive to slightly diminished quality, their correct analysis will be affected, especially if layers of different lengths are also of different quality.

• DNA-Catalyzed Transfer of a Reporter Group

  2006

  Journal of the American Chemical Society · 145 Zitationen · DOI

  Herein we describe a novel DNA-catalyzed transfer of a reporter group from a donating to an accepting oligo-peptide nucleic acid (PNA), generating high catalytic turnover numbers in a DNA-sequence specific manner. The demonstrated transfer of the Dabcyl group was designed to switch on emission of a fluorescein dye while switching off emission of a rhodamine dye. This setup allows the highly sensitive and selective detection of DNA-sequences in real time.

• Large Dynamic Stokes Shift of DNA Intercalation Dye Thiazole Orange has Contribution from a High-Frequency Mode

  2006

  Journal of the American Chemical Society · 137 Zitationen · DOI

  Fluorescence of the cyanine dye Thiazole Orange (TO) is quenched by intramolecular twisting in the excited state. In polypeptide nucleic acids, a vibrational progression in a 1400 cm(-1) mode depends on base pairing, from which follows that the high-frequency displacement is coupled to the twist coordinate. The coupling is intrinsic to TO. This is shown by femtosecond fluorescence upconversion and transient absorption spectroscopy with the dye in methanol solution. Narrow emission from the Franck-Condon state shifts to the red and broadens within 100 fs. The radiative rate does not decrease during this process. Vibrational structure builds up on a 200 fs time scale; it is assigned to asymmetric stretching activity in the methine bridge. Further Stokes shift and decay are observed over 2 ps. Emission from the global S(1) minimum is discovered in an extremely wide band around 12 000 cm(-1). As the structure twists away from the Franck-Condon region, the mode becomes more displaced and overlap with increasingly higher vibrational wave functions of the electronic ground state is achieved. Twisting motion is thus leveraged into a fast-shrinking effective energy gap between the two electronic states, and internal conversion ensues.

• Chemical Properties of Oxidized Silicon Carbide Surfaces upon Etching in Hydrofluoric Acid

  2009

  Journal of the American Chemical Society · 133 Zitationen · DOI

  Hydrogen termination of oxidized silicon in hydrofluoric acid results from an etching process that is now well understood and accepted. This surface has become a standard for studies of surface science and an important component in silicon device processing for microelectronics, energy, and sensor applications. The present work shows that HF etching of oxidized silicon carbide (SiC) leads to a very different surface termination, whether the surface is carbon or silicon terminated. Specifically, the silicon carbide surfaces are hydrophilic with hydroxyl termination, resulting from the inability of HF to remove the last oxygen layer at the oxide/SiC interface. The final surface chemistry and stability critically depend on the crystal face and surface stoichiometry. These surface properties affect the ability to chemically functionalize the surface and therefore impact how SiC can be used for biomedical applications.

• Wound Healing in Mice with High-Fat Diet- or<i>ob</i>Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study

  2010

  Experimental Diabetes Research · 131 Zitationen · DOI

  In the past, the genetically diabetic-obese diabetes/diabetes (db/db) and obese/obese (ob/ob) mouse strains were used to investigate mechanisms of diabetes-impaired wound healing. Here we determined patterns of skin repair in genetically normal C57Bl/6J mice that were fed using a high fat diet (HFD) to induce a diabetes-obesity syndrome. Wound closure was markedly delayed in HFD-fed mice compared to mice which had received a standard chow diet (CD). Impaired wound tissue of HFD mice showed a marked prolongation of wound inflammation. Expression of vascular endothelial growth factor (VEGF) was delayed and associated with the disturbed formation of wound margin epithelia and an impaired angiogenesis in the reduced granulation tissue. Normal wound contraction was retarded and disordered. Wound disorders in obese C57Bl/6J mice were paralleled by a prominent degradation of the inhibitor of NFκB (IκB-α) in the absence of an Akt activation. By contrast to impaired wound conditions in ob/ob mice, late wounds of HFD mice did not develop a chronic inflammatory state and were epithelialized after 11 days of repair. Thus, only genetically obese and diabetic ob/ob mice finally developed chronic wounds and therefore represent a better suited experimental model to investigate diabetes-induced wound healing disorders.

• What is the Barrier for Tunneling Through Alkyl Monolayers? Results from n‐ and p‐Si–Alkyl/Hg Junctions

  2007

  Advanced Materials · 130 Zitationen · DOI

  By combining experimental electron-transport results through an alkane monolayer sandwiched between Si and a metal, photoemission data from the monolayer-on-Si, and theoretical calculations, we show that transport is dominated by a distribution of mixed Si molecular levels, rather than a single molecular level, as shown schematically in the figure. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2089/2007/c1729_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

• Achieving Turnover in DNA‐Templated Reactions

  2008

  ChemBioChem · 124 Zitationen · DOI

  Quasi-intramolecular: Templates bind reactants and increase the effective molarity of their reactive groups. This results in the acceleration of the templated reactions. Programmable self-recognition properties make nucleic acids particularly well adapted to templated synthesis, and have resulted in applications in DNA and RNA detection, prodrug activation and asymmetric catalysis. This minireview aims to summarise the current state of templated reactions catalysed by DNA and RNA.

• Chemoenzymatic Solution- and Solid-Phase Synthesis of<i>O</i>-Glycopeptides of the Mucin Domain of MAdCAM-1. A General Route to<i>O</i>-LacNAc,<i>O</i>-Sialyl-LacNAc, and<i>O</i>-Sialyl-Lewis-X Peptides

  1997

  Journal of the American Chemical Society · 123 Zitationen · DOI

  An efficient and general method for the solid-phase synthesis of glycopeptides containing an O-linked sialyl-Lewis-X (SLex) tetrasaccharide is described. Using a combined chemoenzymatic approach, the first synthesis of an unnatural β-O-linked SLex attached to a partial sequence of the mucin domain of the L-selectin ligand MAdCAM-1, was demonstrated. A resin-bound O-glycoconjugate was synthesized from a new Fmoc-threonine building block which carries an O-unprotected β-linked N-acetylglucoseamine (GlcNAc) moiety. The acid- and base-stable HYCRON-linker enabled the complete removal of all protecting groups on solid phase. Glycosyltransferases were employed to extend the glycan on supported and unsupported O-GlcNAc-octapeptide substrates. The acid- and base-sensitive O-glycopeptides were released under practically neutral conditions, taking advantage of the palladium(0)-catalyzed cleavage of the allylic linkage. Studies toward the selective O-deacetylation of ester-linked glycopeptides possessing O-acetyl-protected carbohydrates are also reported.

• Fluorescence Imaging of Influenza H1N1 mRNA in Living Infected Cells Using Single‐Chromophore FIT‐PNA

  2011

  Angewandte Chemie International Edition · 121 Zitationen · DOI

  A message from the virus was detected by a peptide nucleic acid probe that contains thiazole orange as a fluorescent base surrogate (see picture). The high specificity and biostability of the probes lead to significant improvements to the signal-to-background ratio in imaging the mRNA from the influenza H1N1 virus in living infected cells. Detailed facts of importance to specialist readers are published as ”Supporting Information”. Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.

• Exfoliated graphite nanoplatelets–V2O5 nanotube composite electrodes for supercapacitors

  2011

  Journal of Power Sources · 117 Zitationen · DOI

• Single-Nucleotide-Specific PNA−Peptide Ligation on Synthetic and PCR DNA Templates

  2004

  Journal of the American Chemical Society · 116 Zitationen · DOI

  DNA-directed chemical synthesis has matured into a useful tool with applications such as fabrication of defined (nano)molecular architectures, evolution of amplifiable small-molecule libraries, and nucleic acid detection. Most commonly, chemical methods were used to join oligonucleotides under the control of a DNA or RNA template. The full potential of chemical ligation reactions can be uncovered when nonnatural oligonucleotide analogues that can provide new opportunities such as increased stability, DNA affinity, hybridization selectivity, and/or ease and accuracy of detection are employed. It is shown that peptide nucleic acid (PNA) conjugates, nonionic biostable DNA analogues, allowed the fashioning of highly chemoselective and sequence-selective peptide ligation methods. In particular, PNA-mediated native chemical ligations proceed with sequence selectivities and ligation rates that reach those of ligase-catalyzed oligodeoxynucleotide reactions. Usually, sequence-specific ligations can only be achieved by employing short-length probes, which show DNA affinities that are too low to allow stable binding to target segments in large, double-stranded DNA. It is demonstrated that the PNA-based ligation chemistry allowed the development of a homogeneous system in which rapid single-base mutation analyses can be performed even on double-stranded PCR DNA templates.

• Freie Universität Berlin

  SFB 1449/2: Semi-synthetische Ansätze zur Untersuchung der Funktion von Muzinen (TP C01)

  university

• Leibniz-Forschungsinstitut für Molekulare Pharmakologie

  GRK 2473: Bioaktive Peptide - Innovative Aspekte zur Synthese und Biosynthese

  other

• Technische Universität Berlin

  GRK 2473/1: Bioaktive Peptide – Innovative Aspekte zur Synthese und Biosynthese

  university

• Technische Universität Braunschweig

  SPP 1784: Zelluläre Bildgebung der RNA-C-zu-U-Editierung

  university

• Universität Leipzig

  SPP 1623: Peptidtemplat-gesteuerte Biokonjugation an Proteinen in und auf lebenden Zellen für Untersuchungen von GPCR-Transport und -Kreuzreaktivität

  university

• Universität Potsdam

  GRK 2473: Bioaktive Peptide - Innovative Aspekte zur Synthese und Biosynthese

  university

Name

Prof. Dr. rer. nat. Oliver Seitz

Titel

Prof. Dr. rer. nat.

Fakultät

Mathematisch-Naturwissenschaftliche Fakultät

Institut

Institut für Chemie

Arbeitsgruppe

Organische und Bioorganische Chemie III

Telefon

+49 30 2093-7446

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26.4.2026, 01:12:27