Prof. Dr. Matthew Larkum

Profil

• Active Dendrites and Cortical Associations (ActiveCortex)

  Quelle ↗

  Förderer: Horizon 2020: ERC Advanced Grant Zeitraum: 01/2016 - 12/2020 Projektleitung: Prof. Dr. Matthew Larkum

• Cluster NeuroCure II: Professur für Neuronale Plastizität

  Quelle ↗

  Förderer: DFG Exzellenzinitiative Cluster Zeitraum: 11/2012 - 07/2016 Projektleitung: Prof. Dr. Matthew Larkum

• Dendritische Dynamik und die Wahrnehmungsschwelle

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 09/2014 - 08/2017 Projektleitung: Prof. Dr. Matthew Larkum

• Dendro-somatic Coupling and global neuronal signaling (Cortical Coupling)

  Quelle ↗

  Förderer: Horizon Europe: ERC Advanced Grant Zeitraum: 01/2023 - 12/2027 Projektleitung: Prof. Dr. Matthew Larkum

• Die Funktion und Verbindungen der Neocirtikalen-Schicht 7 und weiterreichende Eingaben zum somatosensorischen Kortex

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 10/2017 - 12/2020 Projektleitung: Prof. Dr. Matthew Larkum

• Einstein Visiting Fellow: Dieter Jaeger

  Quelle ↗

  Förderer: Einstein Visiting Fellow Zeitraum: 01/2018 - 09/2021 Projektleitung: Prof. Dr. Matthew Larkum

• Einstein Visiting Fellowship: Panayiota Poirazi

  Quelle ↗

  Förderer: Einstein Visiting Fellow Zeitraum: 03/2020 - 12/2023 Projektleitung: Prof. Dr. Matthew Larkum

• EU: Context Sensitive Multisensory Object Recognition (HBP)

  Quelle ↗

  Zeitraum: 04/2016 - 03/2018 Projektleitung: Prof. Dr. Matthew Larkum

• EU: From Dendrite to Behaviorge – Fiberoptic Imaging and Optogenetic Manipulation of Dendritic Activity in Behaving Animals (DENDRITE2BEHAVIOR)

  Quelle ↗

  Zeitraum: 06/2013 - 05/2015 Projektleitung: Prof. Dr. Matthew Larkum

• EU: Human Brain Project Specific Grant Agreement 2 (HBP2)

  Quelle ↗

  Förderer: Horizon 2020: Research and Innovation Action (RIA) Zeitraum: 04/2018 - 03/2020 Projektleitung: Prof. Dr. Matthew Larkum

• EU: Human Brain Project Specific Grant Agreement 3 (HBP SGA3)

  Quelle ↗

  Förderer: Horizon 2020: Research and Innovation Action (RIA) Zeitraum: 04/2020 - 09/2023 Projektleitung: Prof. Dr. Matthew Larkum

• EU: Imaging Dendrites Across Wake and Sleep – Fiberoptic Measurements of Calcium Activity in Freely Behaving Animals (PERISLEEP)

  Quelle ↗

  Zeitraum: 09/2012 - 08/2016 Projektleitung: Prof. Dr. Matthew Larkum

• EU: State Dependent Flow of Excitation and Activation of Inhibition in Cortical Columns (Imaging Columns)

  Quelle ↗

  Zeitraum: 08/2014 - 07/2016 Projektleitung: Prof. Dr. Matthew Larkum

• EU: Zelluläre Mechanismen der prädiktiven Verarbeitung und ihre Auswirkungen auf Wahrnehmung (Mechpredpro)

  Quelle ↗

  Zeitraum: 09/2018 - 08/2020 Projektleitung: Prof. Dr. Matthew Larkum

• EXC 2049: Comprehensive Approaches to Neurological and Psychiatric Disorders (NeuroCure)

  Quelle ↗

  Förderer: DFG Exzellenzstrategie Cluster Zeitraum: 01/2019 - 12/2025 Projektleitung: Prof. Andrew Plested, Prof. Dr. Michael Brecht, Prof. Dr. rer. nat. Isabel Dziobek, Prof. Dr. Matthew Larkum, Prof. Dr. Dr. h. c. Peter Hegemann

• Forschungskostenzuschuss von KANAE FOUNDATION

  Quelle ↗

  Zeitraum: 11/2014 - 05/2016 Projektleitung: Prof. Dr. Matthew Larkum

• Functional Role of Feedback in Sensory Representation

  Quelle ↗

  Förderer: Einstein Stiftung Berlin Zeitraum: 07/2017 - 09/2020 Projektleitung: Prof. Dr. Matthew Larkum

• Neuronale Aktivitäten während des Schlafes

  Quelle ↗

  Zeitraum: 12/2011 - 03/2013 Projektleitung: Prof. Dr. Matthew Larkum

• Prospektive Kodierung bei kortikalen Pyramidenzellen

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 02/2015 - 05/2018 Projektleitung: Prof. Dr. Matthew Larkum

• SFB 1315/1: Gedächtnisbildung und -konsolidierung in bestimmten Neuronen des Kortex (TP A04)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2018 - 06/2022 Projektleitung: Prof. Dr. Matthew Larkum

• SFB 1315/1: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2018 - 06/2022 Projektleitung: Prof. Dr. Matthew Larkum

• SFB 1315/2: Die Rolle von mTORC1 und der Proteintranslation bei der Gedächtniskonsolidierung im Neokortex (TP A10)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2022 - 06/2026 Projektleitung: Prof. Dr. Matthew Larkum, Prof. Dr. Marina Mikhaylova

• SFB 1315/2: Die Suche nach Engrammen in einem µStim-basierten Gedächtniskonsolidierungs-Paradigma (TP A04)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2022 - 06/2026 Projektleitung: Prof. Dr. Matthew Larkum

• SFB 1315/2: Infrastruktur für Findable, Accessible, Interoperable, Reusable (FAIR) Daten (TP INF)

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2022 - 06/2026 Projektleitung: Prof. Dr. Matthew Larkum

• SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2022 - 06/2026 Projektleitung: Prof. Dr. Matthew Larkum

• SFB 1315: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  Quelle ↗

  Förderer: DFG Sonderforschungsbereich Zeitraum: 07/2018 - 06/2026 Projektleitung: Prof. Dr. Matthew Larkum

• Zelluläre Mechanismen der Gedächtniskonsolidierung

  Quelle ↗

  Förderer: DFG Sachbeihilfe Zeitraum: 04/2014 - 06/2021 Projektleitung: Prof. Dr. Matthew Larkum

• Österreichische Studiengesellschaft für Kybernetik

  KPT

  48 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%

• EBRAINS

  KPT

  45 Treffer85.0%
  • EU: Human Brain Project Specific Grant Agreement 3 (HBP SGA3)
    K

    85.0%

• Fonden Teknologiradet

  KPT

  55 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%

• Barcelona Supercomputing Center - Centro Nacional de Supercomputation

  KPT

  65 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%
  • The Novel Materials Discovery Laboratory (NoMaD)
    K

    85.0%

• Bernstein Center for Computational Neuroscience Berlin

  KPT

  201 Treffer85.0%
  • DFG-Sachbeihilfe: Aufmerksamkeit und sensorische Integration im aktiven Sehen von bewegten Objekten
    K

    85.0%
  • SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene
    K

    85.0%

• Stichting Centrum voor Wiskunde en Informatica

  KPT

  46 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%

• Fundação D. Anna de Sommer Champalimaud e Dr. Carlos Montez Champalimaud

  KPT

  42 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%

• Fortiss GmbH

  KPT

  53 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%

• Bloomfield Science Museum Jerusalem

  KPT

  54 Treffer85.0%
  • EU: Context Sensitive Multisensory Object Recognition (HBP)
    K

    85.0%

• Zoologischer Garten Berlin AG

  PT

  43 Treffer60.6%
  • Tiere zum Sprechen bringen. Logistik, Wissenschaft, Präsentation
    P

    60.6%

• A new cellular mechanism for coupling inputs arriving at different cortical layers

  1999

  Nature · 1222 Zitationen · DOI

• A cellular mechanism for cortical associations: an organizing principle for the cerebral cortex

  2012

  Trends in Neurosciences · 777 Zitationen · DOI

• Synaptic Integration in Tuft Dendrites of Layer 5 Pyramidal Neurons: A New Unifying Principle

  2009

  Science · 706 Zitationen · DOI

  Tuft dendrites are the main target for feedback inputs innervating neocortical layer 5 pyramidal neurons, but their properties remain obscure. We report the existence of N-methyl-D-aspartate (NMDA) spikes in the fine distal tuft dendrites that otherwise did not support the initiation of calcium spikes. Both direct measurements and computer simulations showed that NMDA spikes are the dominant mechanism by which distal synaptic input leads to firing of the neuron and provide the substrate for complex parallel processing of top-down input arriving at the tuft. These data lead to a new unifying view of integration in pyramidal neurons in which all fine dendrites, basal and tuft, integrate inputs locally through the recruitment of NMDA receptor channels relative to the fixed apical calcium and axosomatic sodium integration points.

• Dendritic action potentials and computation in human layer 2/3 cortical neurons

  2020

  Science · 603 Zitationen · DOI

  The active electrical properties of dendrites shape neuronal input and output and are fundamental to brain function. However, our knowledge of active dendrites has been almost entirely acquired from studies of rodents. In this work, we investigated the dendrites of layer 2 and 3 (L2/3) pyramidal neurons of the human cerebral cortex ex vivo. In these neurons, we discovered a class of calcium-mediated dendritic action potentials (dCaAPs) whose waveform and effects on neuronal output have not been previously described. In contrast to typical all-or-none action potentials, dCaAPs were graded; their amplitudes were maximal for threshold-level stimuli but dampened for stronger stimuli. These dCaAPs enabled the dendrites of individual human neocortical pyramidal neurons to classify linearly nonseparable inputs-a computation conventionally thought to require multilayered networks.

• Active Properties of Neocortical Pyramidal Neuron Dendrites

  2013

  Annual Review of Neuroscience · 459 Zitationen · DOI

  Dendrites are the main recipients of synaptic inputs and are important sites that determine neurons' input-output functions. This review focuses on thin neocortical dendrites, which receive the vast majority of synaptic inputs in cortex but also have specialized electrogenic properties. We present a simplified working-model biophysical scheme of pyramidal neurons that attempts to capture the essence of their dendritic function, including the ability to behave under plausible conditions as dynamic computational subunits. We emphasize the electrogenic capabilities of NMDA receptors (NMDARs) because these transmitter-gated channels seem to provide the major nonlinear depolarizing drive in thin dendrites, even allowing full-blown NMDA spikes. We show how apparent discrepancies in experimental findings can be reconciled and discuss the current status of dendritic spikes in vivo; a dominant NMDAR contribution would indicate that the input-output relations of thin dendrites are dynamically set by network activity and cannot be fully predicted by purely reductionist approaches.

• Active cortical dendrites modulate perception

  2016

  Science · 445 Zitationen · DOI

  Now you feel it, now you don't What determines the detection of a sensory stimulus? To address this question, Takahashi et al. combined in vivo two-photon imaging, electrophysiology, optogenetics, and behavioral analysis in a study of mice. Calcium signals in apical dendrites of pyramidal neurons in the somatosensory cortex controlled the perceptual threshold of the mice's whiskers. Strong reduction of dendritic calcium signaling impaired the perceptual detection threshold so that an identical stimulus could no longer be noticed. Science , this issue p. 1587

• Properties of basal dendrites of layer 5 pyramidal neurons: a direct patch-clamp recording study

  2007

  Nature Neuroscience · 444 Zitationen · DOI

• Dendritic mechanisms underlying the coupling of the dendritic with the axonal action potential initiation zone of adult rat layer 5 pyramidal neurons

  2001

  The Journal of Physiology · 424 Zitationen · DOI

  1. Double, triple and quadruple whole-cell voltage recordings were made simultaneously from different parts of the apical dendritic arbor and the soma of adult layer 5 (L5) pyramidal neurons. We investigated the membrane mechanisms that support the conduction of dendritic action potentials (APs) between the dendritic and axonal AP initiation zones and their influence on the subsequent AP pattern. 2. The duration of the current injection to the distal dendritic initiation zone controlled the degree of coupling with the axonal initiation zone and the AP pattern. 3. Two components of the distally evoked regenerative potential were pharmacologically distinguished: a rapidly rising peak potential that was TTX sensitive and a slowly rising plateau-like potential that was Cd(2+) and Ni(2+) sensitive and present only with longer-duration current injection. 4. The amplitude of the faster forward-propagating Na(+)-dependent component and the amplitude of the back-propagating AP fell into two classes (more distinctly in the forward-propagating case). Current injection into the dendrite altered propagation in both directions. 5. Somatic current injections that elicited single Na(+) APs evoked bursts of Na(+) APs when current was injected simultaneously into the proximal apical dendrite. The mechanism did not depend on dendritic Na(+)-Ca(2+) APs. 6. A three-compartment model of a L5 pyramidal neuron is proposed. It comprises the distal dendritic and axonal AP initiation zones and the proximal apical dendrite. Each compartment contributes to the initiation and to the pattern of AP discharge in a distinct manner. Input to the three main dendritic arbors (tuft dendrites, apical oblique dendrites and basal dendrites) has a dominant influence on only one of these compartments. Thus, the AP pattern of L5 pyramids reflects the laminar distribution of synaptic activity in a cortical column.

• A Top-Down Cortical Circuit for Accurate Sensory Perception

  2015

  Neuron · 392 Zitationen · DOI

• Top-down Dendritic Input Increases the Gain of Layer 5 Pyramidal Neurons

  2004

  Cerebral Cortex · 386 Zitationen · DOI

  The cerebral cortex is organized so that an important component of feedback input from higher to lower cortical areas arrives at the distal apical tufts of pyramidal neurons. Yet, distal inputs are predicted to have much less impact on firing than proximal inputs. Here we show that even weak asynchronous dendritic input to the distal tuft region can significantly increase the gain of layer 5 pyramidal neurons and thereby the output of columns in the primary somatosensory cortex of the rat. Noisy currents injected in ramps at different dendritic locations showed that the initial slope of the frequency-current (f/I) relationship increases with the distance of the current injection from the soma. The increase was due to the interaction of dendritic depolarization with back-propagating APs which activated dendritic calcium conductances. Gain increases were accompanied by a change of firing mode from isolated spikes to bursting where the timing of bursts coded the presence of coincident somatic and dendritic inputs. We propose that this dendritic gain modulation and the timing of bursts may serve to associate top-down and bottom-up input on different time scales.

• Dendritic encoding of sensory stimuli controlled by deep cortical interneurons

  2009

  Nature · 382 Zitationen · DOI

• General Anesthesia Decouples Cortical Pyramidal Neurons

  2020

  Cell · 353 Zitationen · DOI

• Calcium electrogenesis in distal apical dendrites of layer 5 pyramidal cells at a critical frequency of back-propagating action potentials

  1999

  Proceedings of the National Academy of Sciences · 348 Zitationen · DOI

  Action potentials in juvenile and adult rat layer-5 neocortical pyramidal neurons can be initiated at both axonal and distal sites of the apical dendrite. However, little is known about the interaction between these two initiation sites. Here, we report that layer 5 pyramidal neurons are very sensitive to a critical frequency of back-propagating action potentials varying between 60 and 200 Hz in different neurons. Bursts of four to five back-propagating action potentials above the critical frequency elicited large regenerative potentials in the distal dendritic initiation zone. The critical frequency had a very narrow range (10-20 Hz), and the dendritic regenerative activity led to further depolarization at the soma. The dendritic frequency sensitivity was suppressed by blockers of voltage-gated calcium channels, and also by synaptically mediated inhibition. Calcium-fluorescence imaging revealed that the site of largest transient increase in intracellular calcium above the critical frequency was located 400-700 micrometer from the soma at the site for initiation of calcium action potentials. Thus, the distal dendritic initiation zone can interact with the axonal initiation zone, even when inputs to the neuron are restricted to regions close to the soma, if the output of the neuron exceeds a critical frequency.

• NMDA spikes enhance action potential generation during sensory input

  2014

  Nature Neuroscience · 327 Zitationen · DOI

• Cellular Mechanisms of Conscious Processing

  2020

  Trends in Cognitive Sciences · 324 Zitationen · DOI

• The Cellular Basis of GABA _B -Mediated Interhemispheric Inhibition

  2012

  Science · 294 Zitationen · DOI

  Curbing the Other Side The two hemispheres of the brain are connected via the corpus callosum; however, this pathway and its function are still not fully understood. Palmer et al. (p. 989 ) used a combination of optogenetic, calcium-imaging, and electrophysiological methods to investigate the cellular mechanism of interhemispheric inhibition of the firing frequency of neocortical layer 5 pyramidal neurons in rats in vivo and in vitro. They discovered that this form of inhibition involved interneurons in the top layers of the cortex that suppressed active dendritic currents synergistically recruited by back-propagating action potentials. This mechanism depended upon a γ-aminobutyric acid type B receptor–mediated mechanism acting on specific ion channels in the dendrites of pyramidal neurons.

• High <i>I</i> _h Channel Density in the Distal Apical Dendrite of Layer V Pyramidal Cells Increases Bidirectional Attenuation of EPSPs

  2001

  Journal of Neurophysiology · 294 Zitationen · DOI

  Despite the wealth of recent research on active signal propagation along the dendrites of layer V neocortical pyramidal neurons, there is still little known regarding the traffic of subthreshold synaptic signals. We present a study using three simultaneous whole cell recordings on the apical dendrites of these cells in acute rat brain slices to examine the spread and attenuation of spontaneous excitatory postsynaptic potentials (sEPSPs). Equal current injections at each of a pair of sites separated by approximately 500 microm on the apical dendrite resulted in equal voltage transients at the other site ("reciprocity"), thus disclosing linear behavior of the neuron. The mean apparent "length constants" of the apical dendrite were 273 and 446 microm for somatopetal and somatofugal sEPSPs, respectively. Trains of artificial EPSPs did not show temporal summation. Blockade of the hyperpolarization-activated cation current (I(h)) resulted in less attenuation by 17% for somatopetal and by 47% for somatofugal sEPSPs. A pronounced location-dependent temporal summation of EPSP trains was seen. The subcellular distribution and biophysical properties of I(h) were studied in cell-attached patches. Within less than approximately 400 microm of the soma, a low density of approximately 3 pA/microm(2) was found, which increased to approximately 40 pA/microm(2) in the apical distal dendrite. I(h) showed activation and deactivation kinetics with time constants faster than 40 ms and half-maximal activation at -95 mV. These findings suggest that integration of synaptic input to the apical tuft and the basal dendrites occurs spatially independently. This is due to a high I(h) channel density in the apical tuft that increases the electrotonic distance between these two compartments in comparison to a passive dendrite.

• Dendritic Spikes in Apical Dendrites of Neocortical Layer 2/3 Pyramidal Neurons

  2007

  Journal of Neuroscience · 290 Zitationen · DOI

  Layer 2/3 (L2/3) pyramidal neurons are the most abundant cells of the neocortex. Despite their key position in the cortical microcircuit, synaptic integration in dendrites of L2/3 neurons is far less understood than in L5 pyramidal cell dendrites, mainly because of the difficulties in obtaining electrical recordings from thin dendrites. Here we directly measured passive and active properties of the apical dendrites of L2/3 neurons in rat brain slices using dual dendritic-somatic patch-clamp recordings and calcium imaging. Unlike L5 cells, L2/3 dendrites displayed little sag in response to long current pulses, which suggests a low density of I(h) in the dendrites and soma. This was also consistent with a slight increase in input resistance with distance from the soma. Brief current injections into the apical dendrite evoked relatively short (half-width 2-4 ms) dendritic spikes that were isolated from the soma for near-threshold currents at sites beyond the middle of the apical dendrite. Regenerative dendritic potentials and large concomitant calcium transients were also elicited by trains of somatic action potentials (APs) above a critical frequency (130 Hz), which was slightly higher than in L5 neurons. Initiation of dendritic spikes was facilitated by backpropagating somatic APs and could cause an additional AP at the soma. As in L5 neurons, we found that distal dendritic calcium transients are sensitive to a long-lasting block by GABAergic inhibition. We conclude that L2/3 pyramidal neurons can generate dendritic spikes, sharing with L5 pyramidal neurons fundamental properties of dendritic excitability and control by inhibition.

• Coincidence Detection in Pyramidal Neurons Is Tuned by Their Dendritic Branching Pattern

  2003

  Journal of Neurophysiology · 276 Zitationen · DOI

  Neurons display a variety of complex dendritic morphologies even within the same class. We examined the relationship between dendritic arborization and the coupling between somatic and dendritic action potential (AP) initiation sites in layer 5 (L5) neocortical pyramidal neurons. Coupling was defined as the relative reduction in threshold for initiation of a dendritic calcium AP due to a coincident back-propagating AP. Simulations based on reconstructions of biocytin-filled cells showed that addition of oblique branches of the main apical dendrite in close proximity to the soma (d < 140 microm) increases the coupling between the apical and axosomatic AP initiation zones, whereas incorporation of distal branches decreases coupling. Experimental studies on L5 pyramids in acute brain slices revealed a highly significant (n = 28, r = 0.63, P < 0.0005) correlation: increasing the fraction of proximal oblique dendrites (d < 140 microm), e.g., from 30 to 60% resulted on average in an increase of the coupling from approximately 35% to almost 60%. We conclude that variation in dendritic arborization may be a key determinant of variability in coupling (49 +/- 17%; range 19-83%; n = 37) and is likely to outweigh the contribution made by variations in active membrane properties. Thus coincidence detection of inputs arriving from different cortical layers is strongly regulated by differences in dendritic arborization.

• Synaptic clustering by dendritic signalling mechanisms

  2008

  Current Opinion in Neurobiology · 269 Zitationen · DOI

• The GABAB1b Isoform Mediates Long-Lasting Inhibition of Dendritic Ca2+ Spikes in Layer 5 Somatosensory Pyramidal Neurons

  2006

  Neuron · 269 Zitationen · DOI

• Functional Fluorescent Ca2+ Indicator Proteins in Transgenic Mice under TET Control

  2004

  PLoS Biology · 244 Zitationen · DOI

  Genetically encoded fluorescent calcium indicator proteins (FCIPs) are promising tools to study calcium dynamics in many activity-dependent molecular and cellular processes. Great hopes-for the measurement of population activity, in particular-have therefore been placed on calcium indicators derived from the green fluorescent protein and their expression in (selected) neuronal populations. Calcium transients can rise within milliseconds, making them suitable as reporters of fast neuronal activity. We here report the production of stable transgenic mouse lines with two different functional calcium indicators, inverse pericam and camgaroo-2, under the control of the tetracycline-inducible promoter. Using a variety of in vitro and in vivo assays, we find that stimuli known to increase intracellular calcium concentration (somatically triggered action potentials (APs) and synaptic and sensory stimulation) can cause substantial and rapid changes in FCIP fluorescence of inverse pericam and camgaroo-2.

• Active dendritic currents gate descending cortical outputs in perception

  2020

  Nature Neuroscience · 243 Zitationen · DOI

• Supralinear Ca²⁺Influx into Dendritic Tufts of Layer 2/3 Neocortical Pyramidal Neurons<i>In Vitro</i>and<i>In Vivo</i>

  2003

  Journal of Neuroscience · 235 Zitationen · DOI

  Pyramidal neurons in layer 2/3 of the neocortex are central to cortical circuitry, but the intrinsic properties of their dendrites are poorly understood. Here we study layer 2/3 apical dendrites in parallel experiments in acute brain slices and in anesthetized rats using whole-cell recordings and Ca2+ imaging. We find that backpropagation of action potentials into the dendritic arbor is actively supported by Na+ channels both in vitro and in vivo. Single action potentials evoke substantial Ca2+ influx in the apical trunk but little or none in the dendritic tuft. Supralinear Ca2+ influx is produced in the tuft, however, when an action potential is paired with synaptic input. This dendritic supralinearity enables layer 2/3 neurons to integrate ascending sensory input from layer 4 and associative input to layer 1.

• Signaling of layer 1 and whisker-evoked Ca2+ and Na+ action potentials in distal and terminal dendrites of rat neocortical pyramidal neurons in vitro and in vivo.

  2002

  PubMed · 233 Zitationen

  Dendritic regenerative potentials play an important role in integrating and amplifying synaptic inputs. To understand how distal synaptic inputs are integrated and amplified, we made multiple simultaneous (double, triple, or quadruple) and sequential (4-12 paired) recordings from different locations of single tufted layer 5 pyramidal neurons in the cortex in vitro and studied the spatial and temporal properties of their dendritic regenerative potential initial zone. Recordings from the soma and from trunk, primary, secondary, tertiary, and quaternary tuft branches of the apical dendrite of these neurons reveal a spatially restricted low-threshold zone approximately 550-900 microm from the soma for Ca2+-dependent regenerative potentials. Dendritic regenerative potentials initiated in this zone have a clearly defined threshold and a refractory period, and they can propagate actively along the dendrite before evoking somatic action potentials. The detailed biophysical characterization of this dendritic action potential initiation zone allowed for the further investigation of dendritic potentials in the intact brain and their roles in information processing. By making whole-cell recordings from the soma and varied locations along the apical dendrite of 53 morphologically identified layer 5 pyramidal neurons in anesthetized rats, we found that three of the dendritic potentials characterized in vitro could be induced by spontaneous or whisker inputs in vivo. Thus layer 5 pyramidal neurons of the rat neocortex have a spatially restricted low-threshold zone in the apical dendrite, the activation or interaction of which with the axonal action potential initiation zone is responsible for multiple forms of regenerative potentials critical for integrating and amplifying sensory and modulatory inputs.

• Aalto Universität

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Athens University of Economics and Business - Research Center

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Barcelona Supercomputing Center - Centro Nacional de Supercomputation

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Bauhaus-Universität Weimar

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Bergische Universität Wuppertal

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Bernstein Center for Computational Neuroscience Berlin

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  other

• Bloomfield Science Museum Jerusalem

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Cardiff University

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Centre Hospitalier Universitaire Vaudois

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Centre national de la recherche scientifique

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Charité – Universitätsmedizin Berlin

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  university

• Commissariat a l' Energie Atomique et aux Energies Alternatives

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Consiglio Nazionale delle Ricerche

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Consorci Institut d'Investigacions Biomediques August Pi i Sunyer

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Consorzio Interuniversitario Cineca

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Danmarks Tekniske Universitet

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Debreceni Egyetem

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• De Montfort University

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Deutsches Zentrum für Neurodegenerative Erkrankungen e.V.

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  other

• EBRAINS

  EU: Human Brain Project Specific Grant Agreement 3 (HBP SGA3)

  other

• École normale supérieure de Paris

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Ecole Polytechnique federale de Lausanne

  EU: Human Brain Project Specific Grant Agreement 2 (HBP2)

  university

• Eidgenössische Technische Hochschule Zürich

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Ethniko Kai Kapodistriako Panepistimio Athinon

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• European Brain Research Institute Rita Levi-Montalcini

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Fonden Teknologiradet

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Forschungszentrum Jülich GmbH

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  company

• Fortiss GmbH

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  company

• Foundation for Research & Technology - Hellas

  Dendro-somatic Coupling and global neuronal signaling (Cortical Coupling)

  foundation

• Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Freie Universität Berlin

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  university

• Fundação D. Anna de Sommer Champalimaud e Dr. Carlos Montez Champalimaud

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Fundacio Institut de Bioenginyeria de Catalunya

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• FZI Forschungszentrum Informatik

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Hebrew University of Jerusalem

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Heidelberger Institut für Theoretische Studien HITS gGmbH

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Heinrich-Heine-Universität Düsseldorf

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Imperial College of Science, Technology and Medicine

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Institut du Cerveau et de la Moelle Epiniere

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Institute of Experimental Medicine - Hungarian Academy of Sciences

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Institute of Science and Technology Austria

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Institut Jozef Stefan

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Institut National de Recherche Eninformatique et Automatique

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Institut Pasteur

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Institut Suisse de Bioinformatiquefondation ISB

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Italiens Oberstes Gesundheitsinstitut

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Johann Wolfgang Goethe-Universität Frankfurt am Main

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  university

• Karlsruher Institut für Technologie

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Karolinska-Institut

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Katholieke Universiteit Leuven

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• King's College London

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Koninklijke Nederlandse Akademie van Wetenschappen

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Kunglia Tekniska Hoegskolan

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Laboratorio Europeo di Spettroscopie non Lineari

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Leibniz-Forschungsinstitut für Molekulare Pharmakologie

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  other

• Leiden University Medical Center

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Linne Universitetet

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Max-Delbrück-Centrum für Molekulare Medizin

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  other

• Max-Planck-Forschungsstelle für die Wissenschaft der Pathogene

  EXC 2049: Comprehensive Approaches to Neurological and Psychiatric Disorders (NeuroCure)

  other

• Max-Planck-Institut für Ornithologie

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  other

• Medical Research Council

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Medizinische Universität Innsbruck

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Middlesex University Higher Education Corporation

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Nationales Institut für Kernphysik

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Norges Miljo-Og Biovitenskaplige Universitet

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Österreichische Studiengesellschaft für Kybernetik

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Otto-von-Guericke-Universität Magdeburg

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  university

• Politecnico di Torino

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Pompeu Fabra University

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Rey Juan Carlos University

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Rheinisch-Westfälische Technische Hochschule Aachen

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Ruprecht-Karls-Universität Heidelberg

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Sabanci University

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Scuola Normale Superiore

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Scuola Superiore di Studi Universitari e di Perfezionamento Sant'Anna

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Stichting Centrum voor Wiskunde en Informatica

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Stichting Katholieke Universiteit Brabant

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Technische Universität Berlin

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  university

• Technische Universität Darmstadt

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Technische Universität Dresden

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Technische Universität Graz

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Technische Universität München

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Tel Aviv Universität

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• The Chancellor, Masters and Scholars of the University of Oxford

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• The Research Committee of the Technical University of Crete

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• The University Court of the University of Aberdeen

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• The University of Manchester

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• TTY-Saatio

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Uniklinik RWTH Aachen - Universitätsklinikum Aachen

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universidad Autónoma de Madrid

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universidad de Castilla - La Mancha

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universidad de Granada

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universidade do Minho

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universidad Politécnica de Madrid

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Università degli Studi di Milano

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Antwerpen

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Basel

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Bern / Université de Berne

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Bielefeld

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universitat de Barcelona

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Edinburgh

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Glasgow

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Greifswald

  SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene

  university

• Universität Hamburg

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Helsinki

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Leeds

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Oslo

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Pavia - Universita Degli Studi di Pavia

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universitätsklinikum Hamburg - Eppendorf

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  other

• Universität Uppsala

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universität Zürich

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universite d' Aix Marseille

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Université de Bordeaux

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Université de Genève

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Université de Liège

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universiteit Gent

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universiteit Maastricht

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universiteit van Amsterdam

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universite Lyon 1 Claude Bernard

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Universite Pierre et Marie Curie; Universität Pierre und Marie Curie

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• University College London

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• University of Sheffield

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• University of Surrey

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• University of Sussex

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• University of the West of England, Bristol

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Vrije Universität Amsterdam

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  university

• Weizmann-Institut für Wissenschaften

  EU: Context Sensitive Multisensory Object Recognition (HBP)

  research_institute

Name

Prof. Dr. Matthew Larkum

Titel

Prof. Dr.

Fakultät

Lebenswissenschaftliche Fakultät

Institut

Institut für Biologie

Arbeitsgruppe

Neuronale Plastizität

Telefon

+49 30 450-539117

HU-FIS-Profil

Quelle ↗

Zuletzt gescrapt

26.4.2026, 01:08:20