Kerstin Helbig
Profil
Zusammenfassung
Kerstin Helbig entwickelt Konzepte und Infrastrukturen für professionelles Forschungsdatenmanagement in der Wissenschaft. Sie gestaltet Schulungs- und Trainingsangebote für Forschende und Fachleute, um den Umgang mit Forschungsdaten zu verbessern, und arbeitet an der Etablierung von Standards und Best Practices für FAIR-konforme Datenverwaltung in Hochschulen und Forschungsverbünden.
Skills
Stammdaten
Identität, Organisation und Kontakt aus HU-FIS.
Forschungsthemen8
Concept Development for Collaborative Research Data Management Services
Quelle ↗Förderer: Berlin University Alliance (BUA) Zeitraum: 08/2020 - 11/2022 Projektleitung: Kerstin Helbig, Malte Dreyer
FDLink Rahmenbedingungen für Kulturwandel und gemeinsame Servicelandschaft stärken
Quelle ↗Förderer: DFG sonstige Programme Zeitraum: 12/2024 - 04/2028 Projektleitung: Malte Dreyer, Kerstin Helbig
FDNext Weiterentwicklung der Forschungsdatenpraxis: Werkzeuge zur Qualitätsentwicklung für Serviceeinrichtungen in Kooperation mit Fachbereichen und Forschungsverbünden - Teilprojekt Konzepte und Kompetenzen für FD-Rechtsberatung
Quelle ↗Förderer: DFG sonstige Programme Zeitraum: 09/2023 - 07/2025 Projektleitung: Kerstin Helbig, Malte Dreyer
Mögliche Industrie-Partner224
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Publikationen25
Top 25 nach Zitationen — Quelle: OpenAlex (BAAI/bge-m3 embedded für Matching).
Journal of Bacteriology · 215 Zitationen · DOI
Glutathione (GSH) and its derivative phytochelatin are important binding factors in transition-metal homeostasis in many eukaryotes. Here, we demonstrate that GSH is also involved in chromate, Zn(II), Cd(II), and Cu(II) homeostasis and resistance in Escherichia coli. While the loss of the ability to synthesize GSH influenced metal tolerance in wild-type cells only slightly, GSH was important for residual metal resistance in cells without metal efflux systems. In mutant cells without the P-type ATPase ZntA, the additional deletion of the GSH biosynthesis system led to a strong decrease in resistance to Cd(II) and Zn(II). Likewise, in mutant cells without the P-type ATPase CopA, the removal of GSH led to a strong decrease of Cu(II) resistance. The precursor of GSH, gamma-glutamylcysteine (gammaEC), was not able to compensate for a lack of GSH. On the contrary, gammaEC-containing cells were less copper and cadmium tolerant than cells that contained neither gammaEC nor GSH. Thus, GSH may play an important role in trace-element metabolism not only in higher organisms but also in bacteria.
Journal of Bacteriology · 115 Zitationen · DOI
The higher affinity of Cd(2+) for sulfur compounds than for nitrogen and oxygen led to the theoretical consideration that cadmium toxicity should result mainly from the binding of Cd(2+) to sulfide, thiol groups, and sulfur-rich complex compounds rather than from Cd(2+) replacement of transition-metal cations from nitrogen- or oxygen-rich biological compounds. This hypothesis was tested by using Escherichia coli for a global transcriptome analysis of cells synthesizing glutathione (GSH; wild type), gamma-glutamylcysteine (DeltagshB mutant), or neither of the two cellular thiols (DeltagshA mutant). The resulting data, some of which were validated by quantitative reverse transcription-PCR, were sorted using the KEGG (Kyoto Encyclopedia of Genes and Genomes) orthology system, which groups genes hierarchically with respect to the cellular functions of their respective products. The main difference among the three strains concerned tryptophan biosynthesis, which was up-regulated in wild-type cells upon cadmium shock and strongly up-regulated in DeltagshA cells but repressed in DeltagshB cells containing gamma-glutamylcysteine instead of GSH. Overall, however, all three E. coli strains responded to cadmium shock similarly, with the up-regulation of genes involved in protein, disulfide bond, and oxidative damage repair; cysteine and iron-sulfur cluster biosynthesis; the production of proteins containing sensitive iron-sulfur clusters; the storage of iron; and the detoxification of Cd(2+) by efflux. General energy conservation pathways and iron uptake were down-regulated. These findings indicated that the toxic action of Cd(2+) indeed results from the binding of the metal cation to sulfur, lending support to the hypothesis tested.
Biochimica et Biophysica Acta (BBA) - Biomembranes · 17 Zitationen · DOI
Kooperationen8
Bestätigte Forscher↔Partner-Paare aus HU-FIS — Gold-Standard-Positive für das Matching.
FDLink Rahmenbedingungen für Kulturwandel und gemeinsame Servicelandschaft stärken
university
FDLink Rahmenbedingungen für Kulturwandel und gemeinsame Servicelandschaft stärken
university
FDLink Rahmenbedingungen für Kulturwandel und gemeinsame Servicelandschaft stärken
university