Dr. Rucha Kshirsagar

Profil

• Strukturelle und funktionelle Analyse der Interaktion zwischen Kinetochor und zentrometrischem Chromatin in Saccharomyces cerevisiae

  Quelle ↗

  Förderer: DFG Eigene Stelle (Sachbeihilfe) Zeitraum: 10/2018 - 07/2023 Projektleitung: Dr. Rucha Kshirsagar

• Bernstein Center for Computational Neuroscience Berlin

  PT

  7 Treffer53.2%
  • DFG-Sachbeihilfe: Aufmerksamkeit und sensorische Integration im aktiven Sehen von bewegten Objekten
    T

    53.2%
  • SFB 1315/2: Mechanismen und Störungen der Gedächtniskonsolidierung: Von Synapsen zur Systemebene
    P

    53.0%

• Science & Startups Berlin

  T

  1 Treffer51.4%
  • Zuwendung im Rahmen des Programms „exist – Existenzgründungen aus der Wissenschaft“ aus dem Bundeshaushalt, Einzelplan 09, Kapitel 02, Titel 68607, Haushaltsjahr 2026, sowie aus Mitteln des Europäischen Strukturfonds (hier Euro-päischer Sozialfonds Plus – ESF Plus) Förderperiode 2021-2027 – Kofinanzierung für das Vorhaben: „exist Women“
    T

    51.4%

• Asociación Centro de Investigación Cooperativa en Nanociencia – CIC nanoGUNE

  P

  7 Treffer50.5%
  • DYnamic control in hybrid plasmonic NAnopores: road to next generation multiplexed single MOlecule detection
    P

    50.5%

• Special Elementary School for the Deaf and Hard of Hearing of Argyroupolis

  P

  2 Treffer50.0%
  • Promoting Deaf and Hard of Hearing Children's Theory of Mind and Emotion Understanding
    P

    50.0%

• Demotiko Scholeio Lemesou 23 - Agios Spyridona 2

  P

  2 Treffer50.0%
  • Promoting Deaf and Hard of Hearing Children's Theory of Mind and Emotion Understanding
    P

    50.0%

• Ernst-Adolf-Eschke-Schule für Gehörlose

  P

  2 Treffer50.0%
  • Promoting Deaf and Hard of Hearing Children's Theory of Mind and Emotion Understanding
    P

    50.0%

• Ministry of Education and Culture

  P

  2 Treffer50.0%
  • Promoting Deaf and Hard of Hearing Children's Theory of Mind and Emotion Understanding
    P

    50.0%

• ΚΕ.Δ.Δ.Υ. Ν. Ηρακλείου

  P

  2 Treffer50.0%
  • Promoting Deaf and Hard of Hearing Children's Theory of Mind and Emotion Understanding
    P

    50.0%

• novozymess

  P

  7 Treffer47.9%
  • Engineering of New-Generation Protein Secretion Systems
    P

    47.9%

• UCB Celltech

  P

  7 Treffer47.9%
  • Engineering of New-Generation Protein Secretion Systems
    P

    47.9%

• The HORMA domain: an evolutionarily conserved domain discovered in chromatin-associated proteins, has unanticipated diverse functions

  2014

  Gene · 26 Zitationen · DOI

• Probing the Potential Role of Non-B DNA Structures at Yeast Meiosis-Specific DNA Double-Strand Breaks

  2017

  Biophysical Journal · 22 Zitationen · DOI

• N-Terminal Disordered Domain of <i>Saccharomyces cerevisiae</i> Hop1 Protein Is Dispensable for DNA Binding, Bridging, and Synapsis of Double-Stranded DNA Molecules But Is Necessary for Spore Formation

  2013

  Biochemistry · 13 Zitationen · DOI

  The cytological architecture of the synaptonemal complex (SC), a meiosis-specific proteinaceous structure, is evolutionarily conserved among eukaryotes. However, little is known about the biochemical properties of SC components or the mechanisms underlying their roles in meiotic chromosome synapsis and recombination. Functional analysis of Saccharomyces cerevisiae Hop1, a key structural component of SC, has begun to reveal important insights into its function in interhomolog recombination. Previously, we showed that Hop1 is a structure-specific DNA-binding protein, exhibits higher binding affinity for the Holliday junction, and induces structural distortion at the core of the junction. Furthermore, Hop1 promotes DNA condensation and intra- and intermolecular synapsis between duplex DNA molecules. Here, we show that Hop1 possesses a modular domain organization, consisting of an intrinsically disordered N-terminal domain and a protease-resistant C-terminal domain (Hop1CTD). Furthermore, we found that Hop1CTD exhibits strong homotypic as well as heterotypic protein-protein interactions, and its biochemical activities were similar to those of the full-length Hop1 protein. However, Hop1CTD failed to complement the meiotic recombination defects of the Δhop1 strain, indicating that both N- and C-terminal domains of Hop1 are essential for meiosis and spore formation. Altogether, our findings reveal novel insights into the structure-function relationships of Hop1 and help to further our understanding of its role in meiotic chromosome synapsis and recombination.

• Saccharomyces cerevisiae Red1 protein exhibits nonhomologous DNA end–joining activity and potentiates Hop1-promoted pairing of double-stranded DNA

  2017

  Journal of Biological Chemistry · 6 Zitationen · DOI

  Elucidation of the function of synaptonemal complex (SC) in <i>Saccharomyces cerevisiae</i> has mainly focused on <i>in vivo</i> analysis of recombination-defective meiotic mutants. Consequently, significant gaps remain in the mechanistic understanding of the activities of various SC proteins and the functional relationships among them. <i>S. cerevisiae</i> Hop1 and Red1 are essential structural components of the SC axial/lateral elements. Previous studies have demonstrated that Hop1 is a structure-selective DNA-binding protein exhibiting high affinity for the Holliday junction and promoting DNA bridging, condensation, and pairing between double-stranded DNA molecules. However, the exact mode of action of Red1 remains unclear, although it is known to interact with Hop1 and to suppress the spore viability defects of <i>hop1</i> mutant alleles. Here, we report the purification and functional characterization of the full-length Red1 protein. Our results revealed that Red1 forms a stable complex with Hop1 <i>in vitro</i> and provided quantitative insights into their physical interactions. Mechanistically, Red1 preferentially associated with the Holliday junction and 3-way junction rather than with single- or double-stranded DNA with overhangs. Although Hop1 and Red1 exhibited similar binding affinities toward several DNA substrates, the two proteins displayed some significant differences. Notably, Red1, by itself, lacked DNA-pairing ability; however, it potentiated Hop1-promoted intermolecular pairing between double-stranded DNA molecules. Moreover, Red1 exhibited nonhomologous DNA end-joining activity, thus revealing an unexpected role for Red1 in recombination-based DNA repair. Collectively, this study presents the first direct insights into Red1's mode of action and into the mechanism underlying its role in chromosome synapsis and recombination.

• Methylation of CENP-A/Cse4 on arginine 143 and lysine 131 regulates kinetochore stability in yeast

  2023

  Genetics · 5 Zitationen · DOI

  Post-translational modifications on histones are well known to regulate chromatin structure and function, but much less information is available on modifications of the centromeric histone H3 variant and their effect at the kinetochore. Here, we report two modifications on the centromeric histone H3 variant CENP-A/Cse4 in the yeast Saccharomyces cerevisiae, methylation at arginine 143 (R143me) and lysine 131 (K131me), that affect centromere stability and kinetochore function. Both R143me and K131me lie in the core region of the centromeric nucleosome, near the entry/exit sites of the DNA from the nucleosome. Unexpectedly, mutation of Cse4-R143 (cse4-R143A) exacerbated the kinetochore defect of mutations in components of the NDC80 complex of the outer kinetochore (spc25-1) and the MIND complex (dsn1-7). The analysis of suppressor mutations of the spc25-1 cse4-R143A growth defect highlighted residues in Spc24, Ndc80, and Spc25 that localize to the tetramerization domain of the NDC80 complex and the Spc24-Spc25 stalk, suggesting that the mutations enhance interactions among NDC80 complex components and thus stabilize the complex. Furthermore, the Set2 histone methyltransferase inhibited kinetochore function in spc25-1 cse4-R143A cells, possibly by methylating Cse4-K131. Taken together, our data suggest that Cse4-R143 methylation and Cse4-K131 methylation affect the stability of the centromeric nucleosome, which is detrimental in the context of defective NDC80 tetramerization and can be compensated for by strengthening interactions among NDC80 complex components.

• She Writes, She Leads: Women Creators, Characters, and Perceptions in Indian Children's Shows

  2025

  Zenodo (CERN European Organization for Nuclear Research) · DOI

  Abstract: This research aims to address the misrepresentation of women in Indian children’s media. Since media is evolving rapidly as emerging as a big tool for sharing ideas, potrayals and entertainment, it is important for it to ensure that the potrayals of genders are accurate and inspire and empower young minds instead of making them a part of a patriarchal system and perpetuating the same stereotypes of minimal value roles in the modern media scenario. The research encourages and urges modern media to inculcate complexity, depth, and adopt more inclusive narratives for female characters in Indian children’s media. Keywords: Misrepresentation, Stereotypes, Gender Bias, Potrayal, Children,Media

• She Writes, She Leads: Women Creators, Characters, and Perceptions in Indian Children's Shows

  2025

  Zenodo (CERN European Organization for Nuclear Research) · DOI

  Abstract: This research aims to address the misrepresentation of women in Indian children’s media. Since media is evolving rapidly as emerging as a big tool for sharing ideas, potrayals and entertainment, it is important for it to ensure that the potrayals of genders are accurate and inspire and empower young minds instead of making them a part of a patriarchal system and perpetuating the same stereotypes of minimal value roles in the modern media scenario. The research encourages and urges modern media to inculcate complexity, depth, and adopt more inclusive narratives for female characters in Indian children’s media. Keywords: Misrepresentation, Stereotypes, Gender Bias, Potrayal, Children,Media

• A role for β-1,6- and β-1,3-glucans in kinetochore function in <i>Saccharomyces cerevisiae</i>

  2023

  Genetics · DOI

  Chromosome segregation is crucial for the faithful inheritance of DNA to the daughter cells after DNA replication. For this, the kinetochore, a megadalton protein complex, assembles on centromeric chromatin containing the histone H3 variant CENP-A, and provides a physical connection to the microtubules. Here, we report an unanticipated role for enzymes required for β-1,6- and β-1,3-glucan biosynthesis in regulating kinetochore function in Saccharomyces cerevisiae. These carbohydrates are the major constituents of the yeast cell wall. We found that the deletion of KRE6, which encodes a glycosylhydrolase/ transglycosidase required for β-1,6-glucan synthesis, suppressed the centromeric defect of mutations in components of the kinetochore, foremost the NDC80 components Spc24, Spc25, the MIND component Nsl1, and Okp1, a constitutive centromere-associated network protein. Similarly, the absence of Fks1, a β-1,3-glucan synthase, and Kre11/Trs65, a TRAPPII component, suppressed a mutation in SPC25. Genetic analysis indicates that the reduction of intracellular β-1,6- and β-1,3-glucans, rather than the cell wall glucan content, regulates kinetochore function. Furthermore, we found a physical interaction between Kre6 and CENP-A/Cse4 in yeast, suggesting a potential function for Kre6 in glycosylating CENP-A/Cse4 or another kinetochore protein. This work shows a moonlighting function for selected cell wall synthesis proteins in regulating kinetochore assembly, which may provide a mechanism to connect the nutritional status of the cell to cell-cycle progression and chromosome segregation.

• The Elucidation of the Mechanism of Meiotic Chromosome Synapsis in Saccharomyces Cerevisiae : Insights into the Function of Synaptonemal Complex, Hop1 and Red1, Proteins and the Significance of DNA Quadruplex Structures

  2016

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Name

Dr. Rucha Kshirsagar

Titel

Dr.

Fakultät

Lebenswissenschaftliche Fakultät

Institut

Institut für Biologie

Arbeitsgruppe

Molekulare Zellbiologie

Telefon

+49 30 2093-49640

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26.4.2026, 01:08:08