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Dr. Cezary Smaczniak

Lebenswissenschaftliche Fakultät · Institut für Biologie · Pflanzliche Zell- und Molekularbiologie

Projekte

Themen

3D-Rekonstruktion Annotation Bioinformatik Datenanalyse Epigenetik Genregulation Molekularbiologie Netzwerkanalyse Neurowissenschaften Pflanzenphysiologie Proteinstruktur Strukturbiologie

Zusammenfassung

Die Forschung von Dr. Cezary Smaczniak konzentriert sich auf die molekularen Mechanismen der Genregulation, insbesondere die Rolle von MADS-Domänen-Transkriptionsfaktoren bei der Blütenentwicklung und der Differenzierung weiblicher Gameten in Pflanzen. Durch den Einsatz fortschrittlicher Single-Nucleus RNA-Sequenzierung und räumlicher Rekonstruktionsmethoden erstellt er detaillierte 3D-Atlas der zellulären Dynamik und epidermalen Spezialisierung. Seine Arbeit verbindet strukturelle Biologie mit Bioinformatik, um Protein-Protein-Interaktionen und Chromatin-Zugänglichkeit unter Stressbedingungen zu analysieren. Zusätzlich untersucht er neurowissenschaftliche Aspekte der synaptischen Organisation und Mikrotubuli-Ankerung in Neuronen, was die interdisziplinäre Breite seines Forschungsprofils unterstreicht.

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Stammdaten

Identität, Organisation und Kontakt aus HU-FIS.

Dr. Cezary Smaczniak

HU-FIS-Profil ↗

Fakultät: Lebenswissenschaftliche Fakultät
Institut: Institut für Biologie
Arbeitsgruppe: Pflanzliche Zell- und Molekularbiologie
ORCID: 0000-0002-4663-8275
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Forschungsprojekte

Organspezifische DNA-Bindung und Transkriptionsregulation durch floral homöotische Transkriptionsfaktoren
Quelle ↗
Förderer: DFG Sachbeihilfe Zeitraum: 02/2019 - 06/2022 Projektleitung: Dr. Cezary Smaczniak

Publikationen

25Auswahl der wichtigsten Publikationen — Quelle: OpenAlex.

Developmental and evolutionary diversity of plant MADS-domain factors: insights from recent studies
2012
Development · DOI
Members of the MADS-box transcription factor family play essential roles in almost every developmental process in plants. Many MADS-box genes have conserved functions across the flowering plants, but some have acquired novel functions in specific species during evolution. The analyses of MADS-domain protein interactions and target genes have provided new insights into their molecular functions. Here, we review recent findings on MADS-box gene functions in Arabidopsis and discuss the evolutionary history and functional diversification of this gene family in plants. We also discuss possible mechanisms of action of MADS-domain proteins based on their interactions with chromatin-associated factors and other transcriptional regulators.
Characterization of MADS-domain transcription factor complexes in <i>Arabidopsis</i> flower development
2012
Proceedings of the National Academy of Sciences · DOI
Floral organs are specified by the combinatorial action of MADS-domain transcription factors, yet the mechanisms by which MADS-domain proteins activate or repress the expression of their target genes and the nature of their cofactors are still largely unknown. Here, we show using affinity purification and mass spectrometry that five major floral homeotic MADS-domain proteins (AP1, AP3, PI, AG, and SEP3) interact in floral tissues as proposed in the "floral quartet" model. In vitro studies confirmed a flexible composition of MADS-domain protein complexes depending on relative protein concentrations and DNA sequence. In situ bimolecular fluorescent complementation assays demonstrate that MADS-domain proteins interact during meristematic stages of flower development. By applying a targeted proteomics approach we were able to establish a MADS-domain protein interactome that strongly supports a mechanistic link between MADS-domain proteins and chromatin remodeling factors. Furthermore, members of other transcription factor families were identified as interaction partners of floral MADS-domain proteins suggesting various specific combinatorial modes of action.

Target Genes of the MADS Transcription Factor SEPALLATA3: Integration of Developmental and Hormonal Pathways in the Arabidopsis Flower

2009

PLoS Biology · DOI

The molecular mechanisms by which floral homeotic genes act as major developmental switches to specify the identity of floral organs are still largely unknown. Floral homeotic genes encode transcription factors of the MADS-box family, which are supposed to assemble in a combinatorial fashion into organ-specific multimeric protein complexes. Major mediators of protein interactions are MADS-domain proteins of the SEPALLATA subfamily, which play a crucial role in the development of all types of floral organs. In order to characterize the roles of the SEPALLATA3 transcription factor complexes at the molecular level, we analyzed genome-wide the direct targets of SEPALLATA3. We used chromatin immunoprecipitation followed by ultrahigh-throughput sequencing or hybridization to whole-genome tiling arrays to obtain genome-wide DNA-binding patterns of SEPALLATA3. The results demonstrate that SEPALLATA3 binds to thousands of sites in the genome. Most potential target sites that were strongly bound in wild-type inflorescences are also bound in the floral homeotic agamous mutant, which displays only the perianth organs, sepals, and petals. Characterization of the target genes shows that SEPALLATA3 integrates and modulates different growth-related and hormonal pathways in a combinatorial fashion with other MADS-box proteins and possibly with non-MADS transcription factors. In particular, the results suggest multiple links between SEPALLATA3 and auxin signaling pathways. Our gene expression analyses link the genomic binding site data with the phenotype of plants expressing a dominant repressor version of SEPALLATA3, suggesting that it modulates auxin response to facilitate floral organ outgrowth and morphogenesis. Furthermore, the binding of the SEPALLATA3 protein to cis-regulatory elements of other MADS-box genes and expression analyses reveal that this protein is a key component in the regulatory transcriptional network underlying the formation of floral organs.