Dr. Andrea Rupps
Profil
Zusammenfassung
Dr. Andrea Rupps entwickelt Verfahren zur vegetativen Vermehrung von Nadelbäumen durch Gewebekultur und somatische Embryogenese. Sie nutzt molekularbiologische Methoden, um Vermehrungsgut von forstwirtschaftlich wertvollen Baumarten wie Lärchen, Tannen und Douglasien in hoher Qualität und Menge bereitzustellen. Ihre Arbeit verbindet Grundlagenforschung zu Embryogenese-Genen mit praktischen Anwendungen für die Forstwirtschaft und Waldverjüngung.
Skills
Stammdaten
Identität, Organisation und Kontakt aus HU-FIS.
Forschungsthemen3
Bereitstellung von Lärchen-Vermehrungsgut mit hoher Qualität und Diversität zur Erhöhung der waldbaulichen Flexibilität
Quelle ↗Förderer: Bundesministerium für Landwirtschaft, Ernährung und Heimat Zeitraum: 12/2023 - 11/2026 Projektleitung: Dr. Andrea Rupps
Entwicklung und Evaluierung von Verfahren für die effiziente Produktion von forstwirtschaftlich bedeutsamen Klonen von Hybridlärchen und Tannenarten
Quelle ↗Förderer: Bundesministerium für Forschung, Technologie und Raumfahrt Zeitraum: 09/2021 - 10/2025 Projektleitung: Dr. Andrea Rupps
ERA-ForestValue: Entwicklung von Strategien und Technologien zur Nutzung der somatischen Embryogenese zur Intensivierung der Koniferenproduktion durch multivariate Forstwirtschaft
Quelle ↗Förderer: Bundesministerium für Landwirtschaft, Ernährung und Heimat Zeitraum: 04/2019 - 10/2022 Projektleitung: Prof. Dr. sc. nat. Kurt Zoglauer, Dr. Andrea Rupps
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Publikationen11
Top 25 nach Zitationen — Quelle: OpenAlex (BAAI/bge-m3 embedded für Matching).
Planta · 111 Zitationen · DOI
Frontiers in Plant Science · 50 Zitationen · DOI
Although full sequence data of several embryogenesis-related genes are available in conifers, their functions are still poorly understood. In this study, we focused on the transcription factor WUSCHEL-related HOMEOBOX 2 (WOX2) , which is involved in determination of the apical domain during early embryogenesis, and is required for initiation of the stem cell program in the embryogenic shoot meristem of Arabidopsis . We studied the effects of constitutive overexpression of Pinus pinaster WOX2 ( PpWOX2 ) by Agrobacterium -mediated transformation of P. pinaster somatic embryos and Arabidopsis seedlings. Overexpression of PpWOX2 during proliferation and maturation of somatic embryos of P. pinaster led to alterations in the quantity and quality of cotyledonary embryos. In addition, transgenic somatic seedlings of P. pinaster showed non-embryogenic callus formation in the region of roots and subsequently inhibited root growth. Overexpression of PpWOX2 in Arabidopsis promoted somatic embryogenesis and organogenesis in a part of the transgenic seedlings of the first and second generations. A concomitant increased expression of endogenous embryogenesis-related genes such as AtLEC1 was detected in transgenic plants of the first generation. Various plant phenotypes observed from single overexpressing transgenic lines of the second generation suggest some significant interactions between PpWOX2 and AtWOX2 . As an explanation, functional redundancy in the WOX family is suggested for seed plants. Our results demonstrate that the constitutive high expression of PpWOX2 in Arabidopsis and P. pinaster affected embryogenesis-related traits. These findings further support some evolutionary conserved roles of this gene in embryo development of seed plants and have practical implications toward somatic embryogenesis induction in conifers.
Plant Cell Tissue and Organ Culture (PCTOC) · 19 Zitationen · DOI
Abstract Douglas fir ( Pseudotsuga menziesii ) is one of Europe’s most important non-native tree species due to its drought tolerance as well as timber quality and yield. To obtain superior seed from selected parental trees, breeding programs had been established in seed orchards. Douglas fir seed is used as source material for somatic embryogenesis with the aim to select elite genotypes invaluable for clonal mass propagation. To improve given protocols for somatic embryo initiation, we used immature Douglas fir zygotic embryos as explants and abscisic acid (ABA) as plant growth regulator in contrast to the application of auxins and cytokinins. With ABA supplementation, induction frequencies were slightly but in mean higher than with auxin/cytokinin, showing also a strong genotype effect. This offered the possibility to capture SE cultures from otherwise recalcitrant crosses. Furthermore, we observed remarkable differences between the two sets of plant growth regulators concerning the morphological development of the explants, including the absence of non-embryogenic callus by using ABA as inducer. This simplifies the detection of events and the handling of the obtained cultures. Nevertheless, a histological approach suggested, that the same competent cells are addressed by the different hormonal stimulation. Besides, we studied the influence of different points in time of cone harvest, two different basal media and different genetic backgrounds of the explants as well as the maturation ability of the induced embryogenic cultures. In sum, we were able to improve the first steps of somatic embryogenesis and to maintain a significantly higher number of high-value genotypes.
Kooperationen12
Bestätigte Forscher↔Partner-Paare aus HU-FIS — Gold-Standard-Positive für das Matching.
ERA-ForestValue: Entwicklung von Strategien und Technologien zur Nutzung der somatischen Embryogenese zur Intensivierung der Koniferenproduktion durch multivariate Forstwirtschaft
other
Bereitstellung von Lärchen-Vermehrungsgut mit hoher Qualität und Diversität zur Erhöhung der waldbaulichen Flexibilität
company
Entwicklung und Evaluierung von Verfahren für die effiziente Produktion von forstwirtschaftlich bedeutsamen Klonen von Hybridlärchen und Tannenarten
company